Regulatory

Part:BBa_K2180011

Designed by: Sofiane Safi-Stibler   Group: iGEM16_UPMC-Paris   (2016-08-21)


Arsenic Operator - ArsR Binding Site

Biobrick based on a previous biobrick made by the iGEM09_Newcastle Team (2009-10-14) :
https://parts.igem.org/Part:BBa_K174015
We added two restriction sites: SacII before and PacI after the regulatory region in order to use it in our project. They allow us to replace a regulatory region with another regulatory region. This Region allows ArsR to bind to DNA and repress transcription. In the presence of Heavy Metals such as Arsenic or Cadmium transcription is derepressed because of the fixation to ArsR.
Other metalic ions may bind to ArsR.

T--UPMC-Paris--Seq4b.png
The insert can also be confirmed by digestion using a unique restriction site inside the biobrick and checked by electrophoresis migration

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None